Department of Internal Medicine I

The research project of the doctorate aims at gene editing of hereditary heart disease. For this purpose, gene editing
application to the heart will be studied in a variety of small and large animal models. In vitro assays and detailed analysis of
molecular signaling factors crucial for the achievement of correction of genetic mutation by Cas9-based gene editors will be
pursued, before using the most attractive candidates in vivo in murine and porcine hearts. In doing so basic science is
integrated with translational research.

Your responsibilities:
▪ As part of the molecular lab team, you would conduct state-of-the-art -omics and histological analysis of several tissues
collected from test animals. Moreover, the construction of Cas9 based editors is foreseen.

Your profile:
▪ Master (or diploma) in (molecular) biology or biochemistry
▪ Strong interest in cardiovascular biology
▪ Experience or interest in molecular biology methods
▪ You have an above-average engagement and a genuine interest in science

We offer you:
▪ A DFG-, BMBF- and ERC-funded international research group with a well-equipped and scientifically stimulating
environment and a communicative atmosphere
▪ Comprehensive on-the-job training provided by experienced and qualified colleagues
▪ The opportunity to take part in internal and external professional development courses
We look forward to your application and would appreciate your personal presentation!
Please submit your complete application documents by e-mail to Prof. Dr. Christian Kupatt including
▪ a letter of motivation and future perspectives
▪ CV
▪ certificates from college, bachelor, master degrees and
▪ at least one personal certificate, preferably from the advisor of your Master thesis.

Department of Internal Medicine I
Prof. Dr. Christian Kupatt
Klinikum rechts der Isar der Technischen Universität München
Ismaninger Straße 22
81675 München
E-Mail: christian.kupatt@tum.de

If the candidates’ suitability for the position in question is equal, severely disabled applicants shall be given preference.
Interview-related costs can, unfortunately, not be reimbursed

Master thesis in the Carell Group

We are seeking a highly motivated student to undertake a Master’s thesis or an OC-F Internship in the field
of organic chemistry. The project focuses on exploring a prebiotic synthetic route for the synthesis of sugars
as RNA building blocks.
Background. The formose reaction, discovered by Butlerov in 1861, is probably the most important prebiotic
synthetic route for sugars. He synthesised a complex of sugar mixture like aldoses, ketoses by polymerizing
formaldehyde under basic conditions with calcium hydroxide. The reaction starts with formaldehyde, which
can be produced from CO2 and H2O by photolysis. Mechanism of conversion formaldehyde to glycolaldehyde
is so far unknown. In the next step, glycolaldehyde can react with formaldehyde via an aldol condensation to
give the C3 sugar glyceraldehyde. Then this can isomerize to dihydroxyacetone and then next sugars can
be form by other aldol reactions and carbonyl migrations.
Due to low stereo and regioselectivity yield of ribose is less than 1%. Also, ribose is very unstable under the
strong basic conditions in formose reaction so because of these problems there are intensive work to find a
route to ribose production, because due to the simplicity of converting C1 to C6 molecules, it remains of great
role for the prebiotic research field.

Contact: Interested candidates are invited to apply with a CV to Szymon Drewniak (szymon.drewniak@cup.uni-muenchen.de).