Ultra-highfield NMR continues to unveil groundbreaking insights into the molecular mechanisms of biomolecules, emphasizing the critical roles of dynamics and allostery for biological function. This 4D view of structural biology (considering time, in addition to the three spatial coordinates) – further enabled by breakthroughs in cryo-EM, complementary biophysical techniques, e.g. FRET, computational methods and AI – provides unprecedented mechanistic insights empowering breakthrough therapeutic innovations.