Hepatitis B is a very common chronic viral disease that is difficult to cure. The established therapy is based on the use of nucleoside analogous that can control virus replication but are not curative. Interferon alpha may cure hepatitis B, but is accompanied by high side effects as it is applied systemically. The aim of this sub-project is to package interferon-encoding mRNAs into nanoparticles and deliver them directly to HBV-infected livers.