Publications

Here you will find the latest publications from our research cluster. We invite you to use the articles to find out about the latest developments in our research areas.

2024

Genome-scale pan-cancer interrogation of lncRNA dependencies using CasRx

Montero JJ, Trozzo R, Sugden M, Öllinger R, Belka A, Zhigalova E, Waetzig P, Engleitner T, Schmidt-Supprian M, Saur D, Rad R.

Nat Methods. 2024 Apr;21(4):584-596.

Link to publication

Lipo-Xenopeptide Polyplexes for CRISPR/Cas9 based Gene editing at ultra-low dose

Germer J, Lessl AL, Pöhmerer J, Grau M, Weidinger E, Höhn M, Yazdi M, Cappelluti MA, Lombardo A, Lächelt U, Wagner E.

J Control Release. 2024 Jun;370:239-255. doi: 10.1016/j.jconrel.2024.04.037. Epub 2024 Apr 27.

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In Vivo Endothelial Cell Gene Silencing by siRNA-LNPs Tuned with Lipoamino Bundle Chemical and Ligand Targeting

Yazdi M, Pöhmerer J, Hasanzadeh Kafshgari M, Seidl J, Grau M, Höhn M, Vetter V, Hoch CC, Wollenberg B, Multhoff G, Bashiri Dezfouli A, Wagner E.

Small. 2024 Oct;20(42):e2400643. doi: 10.1002/smll.202400643. Epub 2024 Jun 25.

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Dual Effect by Chemical Electron Transfer Enhanced siRNA Lipid Nanoparticles: Reactive Oxygen Species-Triggered Tumor Cell Killing Aggravated by Nrf2 Gene Silencing

Zhang F, Burghardt T, Höhn M, Wagner E.

Pharmaceutics. 2024 Jun 7;16(6):779. doi: 10.3390/pharmaceutics16060779.

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Modulating efficacy and cytotoxicity of lipoamino fatty acid nucleic acid carriers using disulfide or hydrophobic spacers

Steffens RC, Thalmayr S, Weidinger E, Seidl J, Folda P, Höhn M, Wagner E.

Nanoscale. 2024 Jul 25;16(29):13988-14005. doi: 10.1039/d4nr01357c.

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New insights for the development of efficient DNA vaccines

Berger S, Zeyn Y, Wagner E, Bros M.

Microb Biotechnol. 2024 Nov;17(11):e70053. doi: 10.1111/1751-7915.70053.

Link to publication

Strategies and mechanisms for endosomal escape of therapeutic nucleic acids

Grau M, Wagner E.

Curr Opin Chem Biol. 2024 Aug;81:102506. doi: 10.1016/j.cbpa.2024.102506. Epub 2024 Aug 2.

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Dual pH-responsive CRISPR/Cas9 ribonucleoprotein xenopeptide complexes for genome editing

Luo X, Germer J, Burghardt T, Grau M, Lin Y, Höhn M, Lächelt U, Wagner E.

Eur J Pharm Sci. 2024. doi: 10.1016/j.ejps.2024.106983. Epub 2024 Dec 7.

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A comparative analysis of peptide-delivered antisense antibiotics using diverse nucleotide mimics

Ghosh, C., Popella, L., Dhamodharan, V., Jung, J., Dietzsch, J., Barquist, L., Höbartner, C., & Vogel, J.

(2024) RNA (New York, N.Y.), 30(6), 624–643. https://doi.org/10.1261/rna.079969.124

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TCRGenesis: Generation of SIINFEKL-specific T-cell receptor sequences using autoregressive Transformer

Yang An, Felix Drost, Adrian Straub, Annalisa Marsico, Dirk H Busch, Benjamin Schubert.

OpenReview.net, Published: 13 Oct 2024

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Noncoding RNAs in the Vasculature: Basic Mechanisms and Therapeutic Perspectives

Maegdefessel L, Boon RA, Dimmeler S.

Arterioscler Thromb Vasc Biol. 2024 Jan;44(1):3-6.

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Efficient tandem Cu-catalyzed Click synthesis of multi-sugar-modified oligonucleotides

Tölke A.J., Gaisbauer J.F., Gärtner Y.V., Steigenberger B., Holovan A., Streshnev F., Schneider S., Müller M., Carell T.

Angew. Chem. Int. Ed. Engl. 2024, e202405161. doi: 10.1002/anie.202405161.

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Kinetic Referencing Allows Identification of Epigenetic Cytosine Modifications by Single-Molecule Hybridization Kinetics and Superresolution DNA-PAINT Microscopy

Bauer, J.; Reichl, A.; Tinnefeld, P.

ACS nano 2024, 18, 1496–1503.

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DNA Origami Vesicle Sensors with Triggered Single-Molecule Cargo Transfer

Büber, E.; Yaadav, R.; Schröder, T.; Franquelim, H. G.; Tinnefeld, P.

Angew. Chem. Int. Ed. Engl. 2024, e202408295.

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Controlled mechanochemical coupling of anti-junctions in DNA origami arrays

Cole, F.; Pfeiffer, M.; Wang, D.; Schröder, T.; Ke, Y.; Tinnefeld, P.

Nat Commun 2024, 15, 7894.

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Design of Experiments Grants Mechanistic Insights into the Synthesis of Spermine-Containing PBAE Copolymers

Kromer APE, Sieber-Schäfer F, Farfan JB, and Merkel OM

ACS Applied Materials & Interfaces 2024 16 (29), 37545-37554; DOI: 10.1021/acsami.4c06079

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Lysosomal endonuclease RNase T2 and PLD exonucleases cooperatively generate RNA ligands for TLR7 activation

M. Bérouti, K. Lammens, M. Heiss, L. Hansbauer, S. Bauernfried, J. Stöckl, F. Pinci, I. Piseddu, W. Greulich, M. Wang, C. Jung, T. Fröhlich, T. Carell, K.P. Hopfner, V. Hornung

Immunity 2024, 10.1016/j.immuni.2024.04.010

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Gradual evolution of a homo-L-peptide world on homo-D-configured RNA and DNA

E. Węgrzyn, I. Mejdrova, T. Carell

Chem. Sci. 2024, 10.1039/d4sc03384a

Link to publication

Generation of densely labeled oligonucleotides for the detection of small genomic elements

C. Steinek, M. Guirao-Ortiz, G. Stumberger, A.J. Tölke, D. Hörl, T. Carell, H. Harz, H. Leonhardt

Cells Rep. Methods 2024, 10.1016/j.crmeth.2024.100840

Link to publication

Step-by-Step Towards Biological Homochirality – from Prebiotic Randomness To Perfect Asymmetry

I. Mejdrova, E. Węgrzyn, T. Carell

Chem. – Asian J. 2024, 10.1002/asia.202401074

Link to publication

Robust Bisulfite-Free Single-Molecule Real-Time Sequencing of Methyldeoxycytidine Based on a Novel hpTet3 Enzyme,

H. Sahin, R. Salehi, S. Islam, M. Müller, P. Giehr, T. Carell

Angew. Chem. Int. Ed. 2024, 10.1002/anie.202418500

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Endo-bind-n-seq: identifying RNA motifs of RNA binding proteins isolated from endogenous sources

Tiana Nicole Hanelt, Nora Treiber, Thomas Treiber, Gerhard Lehmann, Norbert Eichner, Tamara Rothmeier, Georg Schmid, Robert Reichelt, Federico Zambelli, Giulio Pavesi, Dina Grohmann, Gunter Meister.

Life Sci Alliance. 2024;8(2):e202402782. Published 2024 Dec 2.

Link to publication

X-linked deletion of Crossfirre, Firre, and Dxz4 in vivo uncovers diverse phenotypes and combinatorial effects on autosomes

Tim P. Hasenbein, Sarah Hoelzl, Zachary D. Smith, Chiara Gerhardinger, Marion O. C. Gonner, Antonio Aguilar-Pimentel, Oana V. Amarie, Lore Becker, Julia Calzada-Wack, Nathalia R. V. Dragano, Patricia da Silva-Buttkus, Lillian Garrett, Sabine M. Hölter, Markus Kraiger, Manuela A. Östereicher, Birgit Rathkolb, Adrián Sanz-Moreno, Nadine Spielmann, Wolfgang Wurst, Valerie Gailus-Durner, Helmut Fuchs, Martin Hrabě de Angelis, Alexander Meissner, Stefan Engelhardt, John L. Rinn, Daniel Andergassen.

Nature Communications volume 15, Article number: 10631 (2024)

Link to publication

Efficient Tandem Cu-Catalyzed Click Synthesis of Multi-Sugar-Modified Oligonucleotides

Annika J. Tölke, Julia F. Gaisbauer, Yasmin V. Gärtner, Babara Steigenberger, Anna Holovan, Filip Streshnev, Sabine Schneider, Markus Müller, Thomas Carell.

Angew. Chem. Int. Ed. Engl. 2024, e202405161, doi: 10.1002/anie.202405161
Link to publication

Lipoamino bundle LNPs for efficient mRNA transfection of dendritic cells and macrophages show high spleen selectivity

Franziska Haase, Jana Pöhmerer, Mina Yazdi, Melina Grau, Yanira Zeyn, Ulrich Wilk, Tobias Burghardt, Miriam Höhn, Christoph Hieber, Matthias Bros, Ernst Wagner, Simone Berger.

Eur. J. Pharm. Biopharm. 2024, 95-109, doi: 10.1016/j.ejpb.2023.11.025.
Link to publication

2023

An Aminoisoxazole-Based Proto-RNA

Felix Xu, Stefan Wiedemann, Jonas Feldmann, Sidney Becker and Thomas Carell.

Chem. Europe, 2023, e202300057, doi: 10.1002/ceur.202300057
Link to publication

Trimannose-coupled antimiR-21 for macrophage-targeted inhalation treatment of acute inflammatory lung damage

Christina Beck, Deepak Ramanujam, Paula Vaccarello, Florenc Widenmeyer, Martin Feuerherd, Cho-Chin Cheng, Anton Bomhard, Tatiana Abikeeva, Julia Schädler, Jan-Peter Sperhake, Matthias Graw, Seyer Safi, Hans Hoffmann, Claudia A. Staab-Weijnitz, Roland Rad, Ulrike Protzer, Thomas Frischmuth, Stefan Engelhardt.

Nat. Commun. 2023, 14, 4564. doi: 10.1038/s41467-023-40185-1
Link to publication

Publications

2024

Genome-scale pan-cancer interrogation of lncRNA dependencies using CasRx

Lipo-Xenopeptide Polyplexes for CRISPR/Cas9 based Gene editing at ultra-low dose

In Vivo Endothelial Cell Gene Silencing by siRNA-LNPs Tuned with Lipoamino Bundle Chemical and Ligand Targeting

Dual Effect by Chemical Electron Transfer Enhanced siRNA Lipid Nanoparticles: Reactive Oxygen Species-Triggered Tumor Cell Killing Aggravated by Nrf2 Gene Silencing

Modulating efficacy and cytotoxicity of lipoamino fatty acid nucleic acid carriers using disulfide or hydrophobic spacers

New insights for the development of efficient DNA vaccines

Strategies and mechanisms for endosomal escape of therapeutic nucleic acids

Dual pH-responsive CRISPR/Cas9 ribonucleoprotein xenopeptide complexes for genome editing

A comparative analysis of peptide-delivered antisense antibiotics using diverse nucleotide mimics

TCRGenesis: Generation of SIINFEKL-specific T-cell receptor sequences using autoregressive Transformer

Noncoding RNAs in the Vasculature: Basic Mechanisms and Therapeutic Perspectives

Efficient tandem Cu-catalyzed Click synthesis of multi-sugar-modified oligonucleotides

Kinetic Referencing Allows Identification of Epigenetic Cytosine Modifications by Single-Molecule Hybridization Kinetics and Superresolution DNA-PAINT Microscopy

DNA Origami Vesicle Sensors with Triggered Single-Molecule Cargo Transfer

Controlled mechanochemical coupling of anti-junctions in DNA origami arrays

Design of Experiments Grants Mechanistic Insights into the Synthesis of Spermine-Containing PBAE Copolymers

Lysosomal endonuclease RNase T2 and PLD exonucleases cooperatively generate RNA ligands for TLR7 activation

Gradual evolution of a homo-L-peptide world on homo-D-configured RNA and DNA

Generation of densely labeled oligonucleotides for the detection of small genomic elements

Step-by-Step Towards Biological Homochirality – from Prebiotic Randomness To Perfect Asymmetry

Robust Bisulfite-Free Single-Molecule Real-Time Sequencing of Methyldeoxycytidine Based on a Novel hpTet3 Enzyme,

Endo-bind-n-seq: identifying RNA motifs of RNA binding proteins isolated from endogenous sources

X-linked deletion of Crossfirre, Firre, and Dxz4 in vivo uncovers diverse phenotypes and combinatorial effects on autosomes

Efficient Tandem Cu-Catalyzed Click Synthesis of Multi-Sugar-Modified Oligonucleotides

Lipoamino bundle LNPs for efficient mRNA transfection of dendritic cells and macrophages show high spleen selectivity

2023

An Aminoisoxazole-Based Proto-RNA

Trimannose-coupled antimiR-21 for macrophage-targeted inhalation treatment of acute inflammatory lung damage

Eisbach Bio GmbH unter den 5 deutschen Biotech-Hoffnungen

Eisbach Bio erhält CRPIT Grant zur Förderung neuer Krebsmedikamente

2024 Nobel Prize for microRNA

Reinhart-Koselleck-Grant for Prof. Irmela Jeremias

Proof of Concept Grant for Thomas Carell

Symposium: New Frontier in Chemical Biology

LMU Spin-off RNhale gegründet

Click-Synthese von Zucker-modifizierten Oligonukleotiden

EpiCure gewinnt 2. Runde beim Businessplanwettbewerb

EisbachBio GmbH: Allosterischen Inhibitor erhält FDA-Freigabe